With the rapid advancement of materials science and optoelectronic technology, precise measurement of interfacial stress in materials has become the current research hotspot. Time-domain Brillouin scattering, a non-contact full-optical experimental technique based on ultrafast lasers and picosecond acoustics, is capable of generating and detecting Brillouin oscillations within transparent media due to acousto-optic interactions, demonstrating significant potential in stress analysis. This study aims to delve into the modulation effects of uniaxial stress on Brillouin oscillation frequencies within high borosilicate glass. Firstly, by establishing a Brillouin oscillation measurement system, measurements of Brillouin oscillations under two distinct uniaxial stresses were completed, and the results revealed a trend of decreasing Brillouin oscillation frequency with increasing compressive stress. Subsequently, building on the theoretical expressions of Brillouin oscillation frequencies and precise measurements of experimental signals, a theoretical relationship between stress and Brillouin oscillation frequency was developed, yielding a stress-Brillouin oscillation frequency modulation coefficient.