| 史培荣,梁慧慧,陈显久,陈维毅*.牵张力使大鼠实验性牙齿移动对PTHrP表达的影响[J].实验力学,2013,28(4):497~501 |
| 牵张力使大鼠实验性牙齿移动对PTHrP表达的影响 |
| Influence of Wistar Rat's Experimental Tooth Movement Caused by Tension Force on PTHrP Expression |
| 投稿时间：2012-10-18 修订日期：2013-02-07 |
| DOI：10.7520/1001-4888-12-137 |
| 中文关键词: 牵张力 Wistar大鼠 牙齿移动 正畸 甲状旁腺激素相关蛋白 基因表达 |
| 英文关键词:tension force wistar rat tooth movement orthodontics parathyroid hormone-related protein gene expression |
| 基金项目:本文课题受山西省科技厅国际合作项目(编号：2012081050-1)、山西省科技攻关项目(编号：2011032107-02)资助 |
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| 中文摘要: |
| 通过探讨牵张力对甲状旁腺激素相关蛋白(Parathyroid hormone related protein,PTHrP)表达、分布的影响,为深入了解正畸牙齿移动过程的骨改建机理提供参考,本文使用体重200±20g、6~8W龄的健康雄性Wistar大鼠42只适应性喂养1W后随机分为7组,每组6只。选择每只大鼠的左侧上颌第一磨牙作为实验牙,以左侧上颌切牙作为支抗牙,安装正畸用0.012英寸(inch)步镍钛螺旋拉簧,力值为50g(0.49N)。分别于安装牙齿移动装置后1d、3d、5d、7d、14d、21d处死实验组大鼠,取含左侧上颌第一磨牙及其周围牙槽骨的组织块,常规进行HE染色和SABC法免疫组织化学染色。利用图像分析系统(0BI200高清晰度彩色病理图文分析系统),对每张免疫组化染色后的切片做定量分析。SPSS17.0建立数据库并进行统计学分析,显示在张力区牙周膜及牙槽骨组织中的成骨细胞、成纤维细胞以及间充质细胞PTHrP呈阳性表达,且张力区牙周组织中PTHrP的表达在加力第5天达到高峰。表明 PTHrP牙周组织的改建有一定的调节作用,使张力区与压力区向不同的方向发展。 |
| 英文摘要: |
| The purpose of this study is through exploring the influence of tension force on expression and distribution of PTHrP (Parathyroid hormone related protein) to provide reference for further understanding the bone reconstruction mechanism in orthodontic tooth movement. 42 healthy male Wistar rats with weight range 200±20g and age range 6-8W were selected. After adaptive feeding for 1 week, they were divided into seven groups, 6 Wistars each group. Selecting the first molar tooth in the left upper jaw of each rat as experimental tooth, taking the incisor tooth in the left upper jaw as anchorage tooth, 0.012-inch Ni-Ti spiral spring for orthodontics was mounted with force value 50 g (0.49N) for each rat. Experimental Wistars were killed at 1, 3, 5, 7, 14, 21 days, respectively, after the tooth movement device was mounted. Taking the first molar tooth in the left upper jaw and the tissue block of its surrounding alveolar bone, a routine HE staining and SABC immunohistochemical staining were conducted. Using image analysis system (OBI200 high-sharpness color pathological graphic analysis system) to make a quantitative analysis of each stained slice of immune group and make a statistical analysis with SPSS17.0. In tension-force area, PTHrP of osteoblast, fibroblast and mesenchymal cell in parodontium and alveolar bone is positively expressed and its expression peaks in the 5-th day after force addition. Tension force is able to adjust the reconstruction of parodontal tissue and direct the PTHrP expression in tension area and pressure area to develop in different ways. |
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