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刘永嫚,徐小海,吴尚犬*,张青川*.基于单层荧光颗粒的三维细胞牵引力的研究[J].实验力学,2018,33(6):831~840
基于单层荧光颗粒的三维细胞牵引力的研究
Study on three dimensional cell traction force based on monolayer fluorescent beads
投稿时间: 2018-03-06  最后修改时间: 2018-04-03
DOI:10.7520/1001-4888-18-044
中文关键词:  细胞牵引力  弹性水凝胶  细胞变形测量  数字体积相关
英文关键词:cell traction force  elastic hydrogel  cell deformation measurement  digital volume correlation
基金项目:国家自然科学基金(Nos.11627803, 11472266和11502265)、中国科学院战略性先导科技专项(B类)(XDB22040502)和中央高校基本科研业务费专项资金(WK2480000002)资助
作者单位
刘永嫚 中国科学技术大学近代力学系中国科学院材料力学行为和设计重点实验室 合肥 230027 
徐小海 中国科学技术大学近代力学系中国科学院材料力学行为和设计重点实验室 合肥 230027 
吴尚犬* 中国科学技术大学近代力学系中国科学院材料力学行为和设计重点实验室 合肥 230027 
张青川* 中国科学技术大学近代力学系中国科学院材料力学行为和设计重点实验室 合肥 230027 
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中文摘要:
      细胞与细胞外微环境的力学相互作用对于细胞迁移、增殖和分化起着至关重要的作用。近年来,基于数字图像相关和数字体积相关方法的细胞牵引力研究得到了广泛的开展。然而,传统的基于数字图像相关的方法只能测量面内的变形;传统的基于数字体积相关的方法将荧光标记颗粒置于水凝胶基底内部,扫描层数过多导致图像采集时间长、光毒性增大。本文中,我们结合激光扫描共聚焦技术和数字体积相关方法,将单层荧光颗粒平铺在水凝胶表面,通过共聚焦显微镜采集细胞迁移过程中不同时刻的图像,采用二阶形函数的数字体积相关方法得到细胞-基底界面处的三维位移场和三维牵引力场。该方法采用单层荧光颗粒,不仅有效地降低了多层激光扫描产生的光毒性,还极大地减少了图像采集时间,从而在扫描同一序列图像时,细胞形态变化相对较小,提高了原始图像的准确性。并且,采用二阶形函数可以描述细胞迁移过程中基底产生的弯曲等更复杂形式的一阶形函数无法描述的变形。
英文摘要:
      The mechanical interaction between cell and extracellular microenvironment plays a crucial role in cell migration, proliferation and differentiation. In recent years, the studies on cell traction based on digital image correlation and digital volume correlation have been extensively carried out. However, the traditional method based on digital image correlation can only measure in-plane deformations. The traditional method based on the digital volume correlation embed the fluorescent microspheres inside the hydrogel substrate, which increases scanning layers and leading to long time of image acquiring and strong phototoxicity. In this paper, authors combine laser scanning confocal microscope (LSCM) and digital volume correlation (DVC). The monolayer fluorescent beads are laid on the surface of the hydrogel. The images are collected at different times during cell migration by confocal microscope. Three dimensional (3D) displacement field and three dimensional traction field at the cell-substrate interface are obtained by using digital volume correlation method of second-order shape function. This method not only effectively reduces the phototoxicity induced by multi-layer laser scanning, but also greatly reduces the time of image acquisition. Thus, when scanning the same sequence image, the cell morphology changes relatively little, the accuracy of original image is improved. Moreover, the second order shape function can be used to describe more complex form such as substrate bending in cell migration process, which can not be described by the first order shape function.
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